Factors Affecting the Variance of Copy Numbers in Real-time PCR as Estimated from Calibration Curves
نویسندگان
چکیده
منابع مشابه
Calibration curves for real-time PCR.
BACKGROUND Despite the increasing use of real-time PCR in the diagnosis and management of viral infections, there are no published studies adequately addressing the optimum number of calibrators, the number of replicates of each calibrator, and the frequency with which calibration needs to be repeated. This study was designed to address these issues. METHODS Cycle threshold data (ABI 7700) wa...
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Gene expression is differently regulated in every cell even though the cells are included in the same tissue. For this reason, we need to measure the amount of mRNAs in a single cell to understand transcription mechanism better. However, there are no accurate, rapid and appropriate methods to determine the exact copy numbers of particular mRNAs in a single cell. We therefore developed a procedu...
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ژورنال
عنوان ژورنال: Journal of the Food Hygienic Society of Japan (Shokuhin Eiseigaku Zasshi)
سال: 2009
ISSN: 0015-6426,1882-1006
DOI: 10.3358/shokueishi.50.1